Emfret公司血小板消除抗體R300說(shuō)明書(shū)
Antibodies for Platelet Depletion in Mice
# R300 |
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產(chǎn)品資料 |
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貨號(hào) | R300 |
同種型(isotype) | 大鼠IgG (Wistar) |
含量 | 0.5 mg purified immunoglobulins in PBS, sterile filtered |
包裝 | 1ML |
濃度 | 0.5 mg/ml |
本產(chǎn)品只用于科研，不用于診斷，也不屬于醫(yī)療用品
特異性: This antibody preparation contains a mixture of purified rat monoclonal antibodies directed
against mouse GPIba (CD42b). Targeting of this receptor with divalent IgGs results in profound and
irreversible Fc-independent plaet depletion in mice1,2. Plaet depletion is frequently used to study
the general role of plaets in (patho-) physiology3.
制備 : 抗體是雜交瘤細(xì)胞上清通過(guò)G-Sepharose純化所得，內(nèi)毒素含量小于 0.0025 EU/µg
保存和運(yùn)輸：4度保存和運(yùn)輸，保質(zhì)期為6個(gè)月 ，無(wú)菌保存不含防腐劑，分裝保存-20度至少可以保存一年以上，但請(qǐng)避免反復(fù)凍融
使用方法: 按動(dòng)物體重注射抗體，用 量為2 µg抗體/g動(dòng)物體重，可以參見(jiàn)上圖。如果4 µg抗體/g動(dòng)物體重，可以持續(xù)更長(zhǎng)時(shí)間。注射后60分鐘內(nèi)可以去除血小板95%以上，建議每次注射100-200µL抗體（用無(wú)菌PBS稀釋?zhuān)?。詳情?lián)系靶點(diǎn)科技技術(shù)支持?？梢月?lián)合清除巨噬細(xì)胞（LIPOSOMA，CP-005-005）和中性粒細(xì)胞，咨詢(xún)綜合解決方案。
引用文獻(xiàn):
1. Bergmeier W, Rackebrandt K, Schroder W, Zirngibl H, Nieswandt B. (2000) Structural and functional characterization of the mouse von Willebrand
factor receptor GPIb-IX with novel monoclonal antibodies. Blood. 95:886-93.
2. Nieswandt B, Bergmeier W, Rackebrandt K, Gessner JE, and Zirngibl H. (2000) Identification of critical antigen-specific mechanisms in the
development of immune thrombocytopenic purpura in mice. Blood. 96:2520-27.
3. Elzey BD, Tian J, Jensen RJ et al. (2003) Plaet-mediated modulation of adaptive immunity. A communication link between innate and adaptive
immune compartments. Immunity.19:9-19.
